Analysis of Annulated Sea Snake Venom, Hydrophis Cyanocinctus, Using Liquid Chromatography and MALDI-TOF/TOF
Saeedeh Mosafer Khorjestan,
Seyed Omid Ranei Siadat,
Annulated sea snake, Hydrophis cyanocinctus, venom was investigated using reversed-phase high-performance
liquid chromatography coupled to electrospray mass spectrometry (ESI-MS) and matrix-assisted laser desorption/
ionization time-of-flight mass spectrometry (MALDI-TOF/TOF). MALDI-TOF/TOF identified three compounds (a
short-chain neurotoxin, a long-chain neurotoxin and a basic phospholipase A2); whose molecular weights were estimated
to be 7285, 10838, and 14235 Da, respectively. Moreover, evolutionary relationship of each protein (identity more than
70%) was separately investigated via phylogenetic trees. This study was done as complementary investigation on the
composition of the venom of H. cyanocinctus, the annulated sea snake from the Persian Gulf.
Keywords: Persian Gulf, phospholipase A2, sea snake, three-finger toxin, venom.
Rights & PermissionsPrintExport