Cell viability is only possible due to a dynamic range of essential nucleic acid-protein complex
formation. DNA replication and repair, gene expression, transcription and protein synthesis are
well-known processes mediated by nucleic acids (DNA and RNA) - protein interactions. Novel nucleic
acid- protein complexes have been identified in the past few years aided by the development of numerous
new techniques such as RNA capture or Tandem RNA Affinity Purification (TRAP). However,
the biophysical and biochemical details of these interactions are mostly unknown. Here, we present
three techniques (Electrophoretic Mobility Shift Assays, Microscale Thermophoresis and Surface
Plasmon Resonance) that are commonly used to quantify and characterize DNA-protein and RNA-protein interactions and
discuss their main advantages and limitations.
Keywords: RNA-Protein interaction, DNA-protein interaction, SRP72, SRP RNA, hSSB2, ssDNA, EMSA, Microscale Thermophoresis,
Surface Plasmon Resonance.
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