Title:Optimization and Application of Imprinted Poly(AA-EGDMA) for Solid Phase Extraction of Ciprofloxacin in Artificial Urine
VOLUME: 9 ISSUE: 4
Author(s):Luis D. Marestoni, Ademar Wong, Gustavo T. Feliciano, Mary R.R. Marchi, Cesar R.T. Tarley and Mariar del Pilar Taboada Sotomayo
Affiliation:Institute of Chemistry, São Paulo State University, Prof. Francisco Degni Street, 55, Quitandinha, Araraquara/SP – zipcode 14800-900, Brazil.
Keywords:Ciprofloxacin, molecularly imprinted polymers, optimization, solid phase extraction.
Abstract:Ciprofloxacin is one of the most widely used antibiotics and knowledge of concentration
in the urine is of great significance for pharmacokinetic and environmental studies. In this work, the
Poly (Acrylic Acid – Ethylene Glycol Dimethacrylate) Molecularly Imprinted Polymer was optimized
as a adsorbent in solid phase extraction (MISPE) for Ciprofloxacin. It is desirable because they are
adsorbent materials highly selective to their template molecule. The MIP resulted in maximum
sorption capacities of 282 μmol g-1 in batch studies and in μmol g-1 in the breakthrough curve studies. MISPE was applied
in synthetic urine to test its viability. The limit of detection was 70 ng mL-1. The enrichment factor of the method was
5.32, and recovery in urine ranged from 94.2% to 120.8%. These results showed the applicability of this optimized
MISPE.
