Production of β -cyclodextrin from pH and Thermo Stable Cyclodextrin Glycosyl Transferase, Obtained from Arthrobacter mysorens and Its Evaluation as a Drug Carrier for Irbesartan

Author(s): Y. Rajesh, K. Narayanan, M. Sreenivasa Reddy, Vijaya K. Bhaskar, G. Gautham Shenoy, V. M. Subrahmanyam, J. Venkata Rao.

Journal Name: Current Drug Delivery

Volume 12 , Issue 4 , 2015

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Abstract:

Cyclodextrins (CDs) are carrier molecules produced by cyclization of α-1,4-glucans by Cyclodextrin Glycosyl Transferase (CGTase). These torus shaped molecules have hydrophobic cavity and hydrophilic shell making them useful in pharmaceutical, food, textile, pesticide and cosmetic industries. In this study, culture conditions for the production of CGTase by organism belonging to Arthrobacter genus obtained from a paddy field soil were optimized by single parameter mode. Soluble starch, yeast extract and magnesium sulphate played an important role in CGTase production. Percentage increase in CGTase yield under optimized conditions was 396.77%. The enzyme precipitated by 60% ammonium sulphate was purified using DEAE-sepharose. The molecular weight of the purified protein as determined by SDS-PAGE was 75 kDa. Purified CGTase was thermostable and stable over a wide pH range. Dissolution studies on β -cyclodextrin-Irbesartan complex revealed that β -CDs formed were useful in preparing immediate release oral dosage forms.

Keywords: β-cyclodextrin, cyclodextrin, irbesartan inclusion complex, thermostable CGTase.

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Article Details

VOLUME: 12
ISSUE: 4
Year: 2015
Page: [444 - 453]
Pages: 10
DOI: 10.2174/1567201812666150422163531
Price: $58

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