An Association between MicroRNA-21 Expression and Vitamin D Deficiency in Coronary Artery Disease

Author(s): Barry J. Sheane, Paul Smyth, Kenneth Scott, Raschid Aziz, Mella Buckley, Emer Lodge, Niamh Kiely, Mary Kingston, Eilis McGovern, Martin Healy, J B Walsh, Orla Sheils, Gaye Cunnane.

Journal Name: MicroRNA

Volume 4 , Issue 1 , 2015

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Abstract:

Background: Atherosclerosis-related cardiovascular disease and osteoporosis (OP) occur concurrently and may share a common pathogenesis. Aberrant expression of miR-21 and vitamin D deficiency have been independently linked to the pathogenesis of atherosclerosis and OP. Objectives: To examine the relationship between miR-21 expression and vitamin D in aorta and bone in atherosclerotic disease. Methods: Aorta, internal mammary artery (IMA) and sternal bone samples were collected from patients undergoing coronary artery bypass graft (CABG) surgery. Bone density was measured by dual x-ray absorbtiometry (DXA). MiR-21 was quantified using a two-step reverse transcription-polymerase chain reaction. Results: Ten patients were included for analysis; 5 were vitamin D deficient (<25nmol/L). MiR-21 was expressed at a greater level in aorta compared with the IMA (p = 0.003), and sternal bone (p = 0.002). Expression of miR-21 between the IMA and bone was similar (p = 0.7). A positive correlation between the magnitude of difference (fold-difference) of miR-21 expression between aorta and IMA and CRP (correlation coefficient 0.9, p = 0.009) was found. Vitamin D deficient patients had greater expression of miR-21 in aorta compared with non-deficient patients (p = 0.03). Increasing CRP and vitamin D deficiency were independent predictors of miR-21 expression in aorta. The lower the difference in miR-21 expression between aorta and bone, the lower the bone density. Conclusion: In atherosclerosis, miR-21 is increased in the aorta and associated with vitamin D deficiency. Vitamin D deficiency may influence aberrant miR-21 expression in vasculature and bone contributing to the concurrent development of atherosclerosis and osteoporosis.

Keywords: Atherosclerosis, bone density, coronary artery disease, ischemic heart disease, microRNA, miR-21, vitamin D.

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Article Details

VOLUME: 4
ISSUE: 1
Year: 2015
Page: [57 - 63]
Pages: 7
DOI: 10.2174/2211536604666150414203919

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