Dynamic Expression of MicroRNAs (183, 135a, 125b, 128, 30c and 27a) in the Rat Pilocarpine Model and Temporal Lobe Epilepsy Patients

Author(s): Walid Alsharafi, Bo Xiao.

Journal Name: CNS & Neurological Disorders - Drug Targets
(Formerly Current Drug Targets - CNS & Neurological Disorders)

Volume 14 , Issue 8 , 2015

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Abstract:

Recently, microRNAs (miRNAs) are emerging as new regulators in the pathogenesis of temporal lobe epilepsy (TLE) and playing a major role in the inflammatory and immune processes. The aim of the present study was to evaluate the dynamic expression of brain-specific miR-183 and miR-135a, brain-enriched miR-125b and miR-128 and inflammation-related miR-30c and miR-27a. Status epilepticus evoked by pilocarpine administeration was used to induce epilepsy in rats. Quantitative polymerase chain reaction was performed on rat hippocampus 2 hours, 3 weeks and 2 months following pilocarpine-induced status epilepticus, representing the acute, latent, and chronic phases, respectively. Expression levels were also measured in hippocampus obtained from TLE patients and normal controls. In the rat model, miR-183, miR-135a and miR-125b were detected upregulated during the acute and chronic phases compared to controls, but not during the latent phase. miR-30c and miR-27a were upregulated in the acute and chronic phases of TLE, while in the latent phase miR-30c was downregulated and miR-27a was upregulated. On the other hand, miR-128 showed significantly downregulated in all phases of TLE development. In TLE patients, miR-183, miR- 135a, miR-125b, miR-30c and miR-27a were upregulated, whereas miR-128 was downregulated. Our study revealed upregulation of miR-183, miR-135a and miR-125b in the seizure-related phases and TLE patients, suggesting that all may provide a potential therapeutic approach for the treatment of TLE, whereas the dysregulation of miR-128, miR-30c and miR-27a may suggest different functions during the process of TLE development.

Keywords: Hippocampus, inflammation, microRNAs, status epilepticus, temporal lobe epilepsy.

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Article Details

VOLUME: 14
ISSUE: 8
Year: 2015
Page: [1096 - 1102]
Pages: 7
DOI: 10.2174/1871527314666150317225945
Price: $58

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