Glycolipids: Isolated from Oplismenus burmannii Induce Glucose Uptake in L6-GLUT4myc Myotube Cells

Author(s): Surjeet Verma, Deepti Arha, Akhilesh Kumar Tamrakar, Santosh Kumar Srivastava.

Journal Name: Current Topics in Medicinal Chemistry

Volume 15 , Issue 11 , 2015

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Abstract:

Bioactivity guided separation of combined n-hexane and chloroform extracts of Oplismenus burmannii resulted in the isolation and characterization of five new glycoglycerolipids, (2S)-1,2,6’-tri- O-hexadecanoyl-3-O-β-D-galactopyranosyl glycerol (1a), (2S)-1,2,6’-tri-O-[(9Z,12Z)-octadeca-9,12- dienoyl]-3-O-β-D-galactopyranosyl glycerol (1b), (2S)-1,6’-di-O-[(9Z,12Z)-octadeca-9,12-dienoyl]-3- O-β-D-galactopyranosyl glycerol (2b), (2S)-1,6’-di-O-[(9Z,12Z,15Z)-octadeca-9,12,15-trienoyl]-3-O-β-D-galactopyranosyl glycerol (2c), and (2S)-1,2-di-O-[(9Z,12Z)-octadeca-9,12-dienoyl]-3-O-(6- sulpho-α-D)-quinovopyranosyl glycerol (3b) along with five known glycoglycerolipids (1c, 2a, 3a, 3c and 4), a cerebroside (5), three monoacylglycerols (6a-c) and α-linoleic acid (7). The isolated compounds, 1-5 were in-vitro tested for their antihyperglycemic potential in terms of increase in 2-deoxyglucose uptake in L6-GLUT4myc myotube cells. The results showed that compounds, 1-5 were showing 1.52 (P<0.05), 1.50 (P<0.05), 1.28, 1.49 (P<0.05) and 1.50 (P<0.05) fold increase in the glucose uptake at concentration of 10 μg/mL and 1.71 (P<0.001), 1.74 (P<0.001), 1.50 (P<0.05), 1.76 (P<0.001) and 1.74 (P<0.001) fold increase in the glucose uptake at concentration of 25 μg/mL respectively. However, standard drug Rosiglitazone increases the glucose uptake by 1.59 fold at the concentration of 10μM. Further work on optimization of the anti-diabetic lead is under progress.

Keywords: Cerebroside, Glucose uptake, Glycoglycerolipids, L6-GLUT4myc myotube cells, Rosiglitazone.

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Article Details

VOLUME: 15
ISSUE: 11
Year: 2015
Page: [1027 - 1034]
Pages: 8
DOI: 10.2174/1568026615666150317223112
Price: $58

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