Identification, Purification and Mass Spectrometric Characterization of Two Novel Cyclic Peptides from Clitoria ternatea
Pp. 209-216 (8)
Sudarslal S., Balaram P. and Laladhas K. P.
Kerala has a rich heritage of plant diversity and health traditions. The history of
drug discovery reveals that the ethno-botanical approach is one of the most productive
methods to screen bioactive compounds. Many drugs currently in use were developed after
scientists began to analyze the chemical constituents of plants. Exploration of the medicinal
uses of plants from traditional knowledge coupled to scientific research methodologies may
be able to determine whether such plants provide therapeutically relevant drug candidates
or not. Clitoria ternatea, a plant from the family Fabaceae is a slender, perennial climber
used as an anti-inflammatory agent in folklore medicine. In an effort to identify
biologically active molecules from Clitoria ternatea, we have isolated two novel, disulfide
rich cyclic peptides using reversed phase high performance liquid chromatography (RP
HPLC). Masses of these peptides were measured as 3058.8 Da and 3074.4 Da by an
electrospray ionization mass spectrometer (ESI MS). Partial sequences of the peptides were
assigned through manual de novo sequencing approach using tandem mass spectrometric
data obtained from a matrix assisted laser desorption ionization time of flight mass
spectrometer (MALDI-TOF MS).
Clitoria ternatea, Cyclic Peptide, de novo sequencing, Electrospray,
Molecular Biophysics Unit, Indian Institute of Science, Bangalore, Karnataka, India.