This article review strategies for Candida antarctica lipase B (CALB) production in Pichia
pastoris expression system and the techniques used for separation and purification of CALB. Due to
ease of purification, most researchers prefer to express CALB as extracellular product. Though, CALB
is secreted into growth medium, it is significant to recover target protein with minimal loss. Protein purification
involves sequence of steps and the extent of CALB purification depends on the final application
of the product. Here, cell clarification techniques such as centrifugation, membrane filtration and
chromatographic techniques like ion-exchange, affinity, hydrophobic interaction and gel permeation
are discussed to purify CALB. As immobilized form of CALB offers numerous advantages over free enzyme, various
immobilization techniques have also been discussed.
Keywords: CALB, Candida antarctica, fermentation, lipase B, Pichia pastoris, purification.
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