Abstract
Antithrombin inhibits blood coagulation through the interaction with serine proteases in both intrinsic and extrinsic pathways. In addition, antithrombin also shows anti-inflammatory properties, which are independent of its effects on coagulation. This work shows for the first time the cloning and sequencing of antithrombin from a snake species. This predicted protein is composed by 430 amino acids and presents about 64.5% sequence identity to human antithrombin. Biacore experiments revealed that the binding affinity of Bothrops jararaca snake antithrombin to heparin was ~30 times higher than that of human antithrombin. Furthermore, Bothrops jararaca antithrombin is more effective in preventing acute inflammation induced by carrageenan when compared to human antithrombin. Hence, the results showed herein suggest that Bothrops jararaca antithrombin can play a key role in the control of acute inflammation and that this molecule might be used as a pharmacological tool and as a prototype for drug development.
Keywords: Antithrombin, Bothrops jararaca snake, carrageenan, cloning, heparin affinity, inflammation, surface plasmon resonance.
Protein & Peptide Letters
Title:Cloning, Characterization and Anti-Inflammatory Properties of Bothrops jararaca Snake Antithrombin
Volume: 22 Issue: 5
Author(s): Karen de Morais-Zani, Kathleen F. Grego, Ricardo J.S. Torquato, Caroline S. Silva, Aparecida S. Tanaka and Anita M. Tanaka-Azevedo
Affiliation:
Keywords: Antithrombin, Bothrops jararaca snake, carrageenan, cloning, heparin affinity, inflammation, surface plasmon resonance.
Abstract: Antithrombin inhibits blood coagulation through the interaction with serine proteases in both intrinsic and extrinsic pathways. In addition, antithrombin also shows anti-inflammatory properties, which are independent of its effects on coagulation. This work shows for the first time the cloning and sequencing of antithrombin from a snake species. This predicted protein is composed by 430 amino acids and presents about 64.5% sequence identity to human antithrombin. Biacore experiments revealed that the binding affinity of Bothrops jararaca snake antithrombin to heparin was ~30 times higher than that of human antithrombin. Furthermore, Bothrops jararaca antithrombin is more effective in preventing acute inflammation induced by carrageenan when compared to human antithrombin. Hence, the results showed herein suggest that Bothrops jararaca antithrombin can play a key role in the control of acute inflammation and that this molecule might be used as a pharmacological tool and as a prototype for drug development.
Export Options
About this article
Cite this article as:
Morais-Zani de Karen, Grego F. Kathleen, Torquato J.S. Ricardo, Silva S. Caroline, Tanaka S. Aparecida and Tanaka-Azevedo M. Anita, Cloning, Characterization and Anti-Inflammatory Properties of Bothrops jararaca Snake Antithrombin, Protein & Peptide Letters 2015; 22 (5) . https://dx.doi.org/10.2174/0929866522666150216150704
DOI https://dx.doi.org/10.2174/0929866522666150216150704 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Hydroxytyrosol and Derivatives: Isolation, Synthesis, and Biological Properties
Current Organic Chemistry Immobilization of Proteins in Ormosil Gels: Functional Properties and Applications
Current Organic Chemistry Supertargeted Chemistry: Identifying Relationships Between Molecular Structures and their Sub-Cellular Distribution
Current Topics in Medicinal Chemistry Learning from Multiple Classifier Systems: Perspectives for Improving Decision Making of QSAR Models in Medicinal Chemistry
Current Topics in Medicinal Chemistry Liver-Based In Vitro Technologies for Drug Biotransformation Studies - A Review
Current Drug Metabolism Labeling of Fluorescent Probes to Albumin Microspheres and B16 Melanoma Extra-Cellular Antigen
Current Drug Delivery Biological Activities of Salvia santolinifolia Boiss. A Multifunctional Medicinal Plant
Current Bioactive Compounds Inhibitors of Nitrogen Oxide Species Production in Animal Models of Inflammation and Future Directions for Therapy in Inflammatory Disorders
Current Medicinal Chemistry - Anti-Inflammatory & Anti-Allergy Agents Folic Acid Conjugated Chitosan Nanoparticles for Tumor Targeting of Therapeutic and Imaging Agents
Pharmaceutical Nanotechnology Curcumin - Properties, Applications and Modification of Structure
Mini-Reviews in Organic Chemistry MT1-MMP Activation of TGF-β Signaling Enables Intercellular Activation of an Epithelial-mesenchymal Transition Program in Cancer
Current Cancer Drug Targets Advances of Liquid Chromatographic Determination of Fumonisins; Potential Mycotoxins for Humans
Current Pharmaceutical Analysis Rare Earth Doped Hydroxyapatite Nanoparticles for In Vitro Bioimaging Applications
Current Physical Chemistry Therapeutic and Diagnostic Applications of Nanoparticles
Current Drug Targets Computational Models and Methods for Drug Target Prediction and Drug Repositioning
Combinatorial Chemistry & High Throughput Screening Trans-3-Phenyl-2-Propenoic Acid (Cinnamic Acid) Derivatives: Structure-Activity Relationship as Hepatoprotective Agents
Medicinal Chemistry A Simple Scoring Model Predicting the Outcome of COVID-19 Patients: Tanta COVID Score
Endocrine, Metabolic & Immune Disorders - Drug Targets Probiotics as a Tool to Biosynthesize Metallic Nanoparticles: Research Reports and Patents Survey
Recent Patents on Drug Delivery & Formulation Bamboo a Supplement to Human Health: A Comprehensive Review on its Ethnopharmacology, Phytochemistry, and Pharmacological Activity
The Natural Products Journal Validation of Doxepin Quantitative Determination Methods for their Application to <i>In Vitro</i>, <i>Ex Vivo</i> and <i>In Vivo</i> Studies
Current Pharmaceutical Analysis