Background: Rate-limiting enzymes of purine and pyrimidine catabolisms might possess
with the abilities of key regulators of cells proliferation. In our previous work we have shown that allopurinol,
classical inhibitor of Xanthine Oxidase (XO; EC. 126.96.36.199), is capable of regulating purine
catabolism in the liver tissue. Our current results prove that evidence for the brain tissue after utility of
other biomolecules – pyridoxine, epinephrine. Methods: Cultivation of (E90) human brain cells was
performed by the modified method of Mattson (1990). Specific activity of XO was delineated by the
utility of the method measuring formation of the uric acid. Quantification of the cells stained by the
Trypan Blue allowed determination of the healthy cells yield. Purification of the XO was performed based on the routine
biochemical procedures with further utility of preparative electrophoresis and RP-HPLC. Results and conclusion: The
newly developed methods for purification of XO served as a basis for delineation of Ki (0.0076 mM), Vmax (0.0279) values
for the pyridoxine as an inhibitor of XO. We studied interaction of XO and pyridoxine in pyridoxine depleted, theophylline
treated rats. Also, we estimated the tight interaction between the synthesis of epinephrine and XO activity, which
greatly influences the processes of cells proliferation and death during in vitro studies.
Keywords: Brain, cell culture, epinephrine, pyridoxine, theophylline, xanthine oxidase.
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