Applications of NMR Spectroscopy

Volume: 1

Indexed in: Scopus, Book Citation Index, Science Edition, EBSCO.

Applications of NMR Spectroscopy is an eBook series devoted to publishing the latest advances in the application of nuclear magnetic resonance (NMR) spectroscopy in practical situations. In the first ...
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Protein-Inhibitor Interaction Studies Using NMR

Pp. 143-181 (39)

Rieko Ishima

Abstract

Solution-state NMR has been widely applied to determine the threedimensional structure, dynamics, and molecular interactions of proteins. The designs of experiments used in protein NMR differ from those used for small-molecule NMR, primarily because the information available prior to an experiment, such as molecular mass and knowledge of the primary structure, is unique for proteins compared to small molecules. In this review article, protein NMR for structural biology is introduced with comparisons to small-molecule NMR, such as descriptions of labeling strategies and the effects of molecular dynamics on relaxation. Next, applications for protein NMR are reviewed, especially practical aspects for protein-observed ligand-protein interaction studies. Overall, the following topics are described: (1) characteristics of protein NMR, (2) methods to detect protein-ligand interactions by NMR, and (3) practical aspects of carrying out protein-observed inhibitor-protein interaction studies.

Keywords:

Drug, inhibitor, interaction, NMR, protein, relaxation, structure.

Affiliation:

Department of Structural Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15260, USA.