In this investigation, the screening of two furanocoumarins; 5,8-
dimethoxypsoralen (1) and heraclinin (2), isolated from the methanol root-extracts of
Tamus communis L for their antioxidant activity and xanthine oxidase inhibitory effect
was carried out, using different assays such as DPPH free radical scavenging effect, -
carotene / linoleic acid, xanthine oxidase (XO) inhibition and in addition to blood total antioxidant capacity. Results revealed
that the two compounds have significant DPPH radical scavenging activity and effective inhibition of linoleic acid
oxidation in a dose-dependent manner; 5,8-dimethoxypsoralen exhibited the highest activity with an I% = 72.69 ± 1.88 %.
These results indicate that the isolated compounds inhibit xanthine oxidase activity and scavenge superoxide radicals with
heraclinin (2) as the more potent xanthine oxidase inhibitor, and 5,8-dimethoxypsoralen (1) as the more effective on cytochrome
c reduction, the two tested compounds can effectively protect erythrocytes against hemolytic injury induced by
AAPH. These results are promising for further studies of the biological and pathological effects of these natural products.
Keywords: Antioxidant activity, furanocoumarins, polyphenols, scavenging, Tamus communis L., xanthine oxidase, hemolysis.
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