Objective: To better understand the inflammatory response in the central nervous system
(CNS) after lipopolysaccharide (LPS)-induced chorioamnionitis.
Study Design: Fetal sheep were exposed to intra-amniotic LPS 2 or 14 days before preterm delivery
at 125 days of gestation. mRNA levels of cytokines, TLRs and anti-oxidants were determined in
different CNS regions.
Results: Interleukin 1β levels increased in hippocampus, cortex and cerebellum 2 days after LPS
exposure, while Interleukin 8 levels increased in the periventricular white matter as well. Levels
returned back to control levels after 14 days. Tumor necrosis factor-α levels increased in
hippocampus and cortex after 2 days. Toll like receptor 4 levels was upregulated in all grey matter
regions 2 and 14 days after exposure. Glutathione s-transferase mRNA levels were lower after 2 and 14 days in all grey
Conclusion: Intra-amniotic LPS exposure causes acute and region-specific changes in inflammatory markers in the fetal
brain, with grey matter being more affected than white matter.
Condensation: Intra-amniotic LPS exposure causes acute and region-specific changes in cytokines, TLR and anti-oxidants
levels, with grey matter being more affected than white matter.