In this study, a capillary electrophoresis-mass spectrometry method was applied to the determination
of isoflavones in samples with low content in these analytes, such as legumes. Sample stacking was
used to enhance the sensitivity of the technique. Extraction of the analytes was carried out by a modified
approach of QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) methodology, in which the
extracts were injected directly into electrophoretic system coupled with mass spectrometry detector. The
developed method was validated and applied for the determination of isoflavone aglycones and glycosides in legume
samples. The values of the validation parameters and the isoflavone contents found were compared with those obtained by
LC-MS/MS. Both methods provided similar results and were comparable in terms of recovery (in the 80-120% range),
precision (lower than 10 %) and limits of detection (ranging from 0.20 to 1.8 μg L-1).
Keywords: CE-ESI-MS, LC-ESI-MS/MS, Isoflavone, legume, QuEChERS.
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