Determination of Tigecycline by Quantum Dots/Gold Nanoparticles-based Fluorescent and Colorimetric Sensing System
In this study, two simple and sensitive methods for detection of tigecycline are developed based
on thioglycolic acid-capped CdTe quantum dots (TGA-CdTe QDs) and unmodified gold nanoparticles
(AuNPs), respectively. The first method was based on the fluorescence quenching of CdTe QDs by the
addition of tigecycline. For the second method, the introduction of tigecycline could induce the aggregation
of gold nanoparticles, displaying distinct changes in color and in UV-vis spectra. Under optimal conditions,
the relative fluorescence intensity of CdTe QDs decreased linearly with the increasing concentration
of tigecycline in the range of 0.11-35.56 ug mL-1 with a detection limit of 3.93×10-2 ug mL-1. While the colorimetric
sensor could detect tigecycline in linear range from 5.98×10-3 to 1.44×10-1 ug mL-1 and from 0.30 to 2.99 ug mL-1 with a
detection limit of 1.07×10-3 ug mL-1. Moreover, both the proposed methods were applied to real sample with satisfactory
results. Finally, comparisons between these two methods were made.
Keywords: Colorimetric, fluorescence quenching, gold nanoparticles, quantum dots, tigecycline.
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