Nanodiscs are disc-like structures formed by two copies of a membrane scaffold protein, engineered
from apolipoprotein A-I, surrounding a phospholipid mixture that can incorporate membrane proteins
preserving their natural properties. They behave as soluble entities allowing the use of high-resolution structural
techniques to determine the structural organization of the embedded membrane protein, and the use of
solution biochemical-biophysical tools to measure its activity, assembly and interactions with other proteins in membranelike
environments. In addition, nanodiscs are biocompatible which makes them an attractive technology to be used in
therapy, drug discovery, and other biotechnological applications.
Keywords: Biochemistry, Biophysics, Membrane-like systems, Membrane proteins, Membrane reconstitution, Protein-lipid
interactions, Protein-protein interactions, Structural biology.
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