Abstract
Nanodiscs are disc-like structures formed by two copies of a membrane scaffold protein, engineered from apolipoprotein A-I, surrounding a phospholipid mixture that can incorporate membrane proteins preserving their natural properties. They behave as soluble entities allowing the use of high-resolution structural techniques to determine the structural organization of the embedded membrane protein, and the use of solution biochemical-biophysical tools to measure its activity, assembly and interactions with other proteins in membranelike environments. In addition, nanodiscs are biocompatible which makes them an attractive technology to be used in therapy, drug discovery, and other biotechnological applications.
Keywords: Biochemistry, Biophysics, Membrane-like systems, Membrane proteins, Membrane reconstitution, Protein-lipid interactions, Protein-protein interactions, Structural biology.
Current Topics in Medicinal Chemistry
Title:Phospholipid Bilayer Nanodiscs: A Powerful Tool to Study the Structural Organization and Biochemical Reactivity of Proteins in Membrane-like Environments
Volume: 14 Issue: 23
Author(s): Víctor M. Hernandez-Rocamora, Concepcion Garcia-Montanes and German Rivas
Affiliation:
Keywords: Biochemistry, Biophysics, Membrane-like systems, Membrane proteins, Membrane reconstitution, Protein-lipid interactions, Protein-protein interactions, Structural biology.
Abstract: Nanodiscs are disc-like structures formed by two copies of a membrane scaffold protein, engineered from apolipoprotein A-I, surrounding a phospholipid mixture that can incorporate membrane proteins preserving their natural properties. They behave as soluble entities allowing the use of high-resolution structural techniques to determine the structural organization of the embedded membrane protein, and the use of solution biochemical-biophysical tools to measure its activity, assembly and interactions with other proteins in membranelike environments. In addition, nanodiscs are biocompatible which makes them an attractive technology to be used in therapy, drug discovery, and other biotechnological applications.
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Hernandez-Rocamora M. Víctor, Garcia-Montanes Concepcion and Rivas German, Phospholipid Bilayer Nanodiscs: A Powerful Tool to Study the Structural Organization and Biochemical Reactivity of Proteins in Membrane-like Environments, Current Topics in Medicinal Chemistry 2014; 14 (23) . https://dx.doi.org/10.2174/1568026614666141215142951
DOI https://dx.doi.org/10.2174/1568026614666141215142951 |
Print ISSN 1568-0266 |
Publisher Name Bentham Science Publisher |
Online ISSN 1873-4294 |
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