Metabolism of three different agro-pesticides widely used in Uruguay, the insecticides imidacloprid and
thiamethoxam and the antiparasite nitroxinil, by bovine ruminal fluid, as supply of anaerobic microorganims, was studied.
Complete ruminal fluid was incubated with each of the agrochemicals in different conditions, varying time, nutrients, and
nitroethane supplementation as methanogenesis modificator. Only biotransformation was detected for nitroxinil in some
of the studied variables. In the optimized condition only one product was generated and the chemical structure of this
main metabolite was elucidated using combined spectroscopies evidencing a structural motive unrelated with the products
of the corresponding mammal biotransformation results of reduction, and substitution processes. The ruminal generation
of the metabolite was confirmed. In order to employ this anaerobic microbial system as potential bioremediator of
agrochemical-contaminated soils, the toxicity, against mammal cells, and the mutagenicity, using Ames test, of the
product of biotransformation were studied. The lack of toxic effects encouraged us to propose the ruminal system as a
plausible system for agrochemicals bioremediation.