Abstract
Some phenylalanine (Phe) derivatives had important roles in pharmacology and may be used as pharmaceutical materials and pharmaceutical intermediates. In order to understand the cell toxicity of phenylalanine derivatives, we synthesized L-4-bromo-phenylalanine (Brp), L-4-iodophenylalanine (Ip), L-4-nitro-phenylalanine (Np), L-4-sulfonic-phenylalanine (Sp), L-4-phosphophenylalanine (Pp) and L-4-amino-phenylalanine (Np). We used mass spectrometry (MS), Infrared Spectroscopy (IR) or hydrogen-1 nuclear magnetic resonance spectrum (1H-NMR), and high performance liquid chromatography (HPLC) to test the correction of these products, MTT assay and Hoechst33258 staining to detect their cell toxic effect on human colon cancer cell HT-29 (HT-29 cells). The results showed that these products were correct and the cytotoxicity of PpIpSp and NpBrp, Ap almost had no effect on HT-29 cells. In addition, Pp, Ip and Sp induced cell apoptosis, the other three kinds of phenylalanine derivatives induced neither apoptosis nor necrosis.
Keywords: Phenylalanine, L-4-phenylalanine derivatives, human colon cancer cell line HT-29 (HT-29 cells), median lethal dose (LD50), cell toxicity, cell apoptosis.
Letters in Drug Design & Discovery
Title:Synthesis of Six Phenylalanine Derivatives and their Cell Toxicity Effect on Human Colon Cancer Cell Line HT-29
Volume: 12 Issue: 6
Author(s): Qianyi Zhao, Ting Xu, Menghua Li, Ying Yang, Haopeng Hu, Shupei Wang, Wenjuan Yan, Ran Chen, Chunyan Zhang and Cunshuan Xu
Affiliation:
Keywords: Phenylalanine, L-4-phenylalanine derivatives, human colon cancer cell line HT-29 (HT-29 cells), median lethal dose (LD50), cell toxicity, cell apoptosis.
Abstract: Some phenylalanine (Phe) derivatives had important roles in pharmacology and may be used as pharmaceutical materials and pharmaceutical intermediates. In order to understand the cell toxicity of phenylalanine derivatives, we synthesized L-4-bromo-phenylalanine (Brp), L-4-iodophenylalanine (Ip), L-4-nitro-phenylalanine (Np), L-4-sulfonic-phenylalanine (Sp), L-4-phosphophenylalanine (Pp) and L-4-amino-phenylalanine (Np). We used mass spectrometry (MS), Infrared Spectroscopy (IR) or hydrogen-1 nuclear magnetic resonance spectrum (1H-NMR), and high performance liquid chromatography (HPLC) to test the correction of these products, MTT assay and Hoechst33258 staining to detect their cell toxic effect on human colon cancer cell HT-29 (HT-29 cells). The results showed that these products were correct and the cytotoxicity of PpIpSp and NpBrp, Ap almost had no effect on HT-29 cells. In addition, Pp, Ip and Sp induced cell apoptosis, the other three kinds of phenylalanine derivatives induced neither apoptosis nor necrosis.
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Cite this article as:
Zhao Qianyi, Xu Ting, Li Menghua, Yang Ying, Hu Haopeng, Wang Shupei, Yan Wenjuan, Chen Ran, Zhang Chunyan and Xu Cunshuan, Synthesis of Six Phenylalanine Derivatives and their Cell Toxicity Effect on Human Colon Cancer Cell Line HT-29, Letters in Drug Design & Discovery 2015; 12 (6) . https://dx.doi.org/10.2174/1570180812666141206001604
DOI https://dx.doi.org/10.2174/1570180812666141206001604 |
Print ISSN 1570-1808 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-628X |
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