Abstract
Paedocypris is a newly described minifish genus endemic to Southeast Asia. Besides a tiny adult size of ~8 mm in length, minifish feature fragmentary habitats of acidic peat blackwater swamps, an unusual reproduction mode, truncated development and one of the smallest known genomes. A complete sequence is absent for the minifish mitochondrial DNA (mtDNA). Here we report the complete mtDNA sequence and its unusual feature in the minifish P. progenetica (Pp). We show that the Pp mtDNA is a circular molecule of 17,382 bp in length and has the same number of similarly oriented genes as in other vertebrates. Specifically, it comprises 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes and 1 D-loop. Surprisingly, the D-loop is elusive for amplification by standard PCR conditions. The D-loop possesses a 28-bp dinucleotide TA repeat and more intriguingly, up to 25 copies of a 34-bp tandem repeat sequence. These tandem repeats predict the formation of paired regions. Hence, besides a generally conserved mtDNA with other vertebrates, the Pp mtDNA features an unusual D-loop and compromised DNA replication in vitro.
Keywords: Control region, D-loop, minifish, mitogenome, mtDNA.
Current Molecular Medicine
Title:Minifish mtDNA has Abundance of Repeat Sequences and Inefficient Replication In Vitro
Volume: 14 Issue: 10
Author(s): J. Wang, L.-Y. Peng, C.-P. You, Q.-L. Li, M. Wen, S.-J. Liu and Y.-H. Hong
Affiliation:
Keywords: Control region, D-loop, minifish, mitogenome, mtDNA.
Abstract: Paedocypris is a newly described minifish genus endemic to Southeast Asia. Besides a tiny adult size of ~8 mm in length, minifish feature fragmentary habitats of acidic peat blackwater swamps, an unusual reproduction mode, truncated development and one of the smallest known genomes. A complete sequence is absent for the minifish mitochondrial DNA (mtDNA). Here we report the complete mtDNA sequence and its unusual feature in the minifish P. progenetica (Pp). We show that the Pp mtDNA is a circular molecule of 17,382 bp in length and has the same number of similarly oriented genes as in other vertebrates. Specifically, it comprises 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes and 1 D-loop. Surprisingly, the D-loop is elusive for amplification by standard PCR conditions. The D-loop possesses a 28-bp dinucleotide TA repeat and more intriguingly, up to 25 copies of a 34-bp tandem repeat sequence. These tandem repeats predict the formation of paired regions. Hence, besides a generally conserved mtDNA with other vertebrates, the Pp mtDNA features an unusual D-loop and compromised DNA replication in vitro.
Export Options
About this article
Cite this article as:
Wang J., Peng L.-Y., You C.-P., Li Q.-L., Wen M., Liu S.-J. and Hong Y.-H., Minifish mtDNA has Abundance of Repeat Sequences and Inefficient Replication In Vitro, Current Molecular Medicine 2014; 14 (10) . https://dx.doi.org/10.2174/1566524014666141202163927
DOI https://dx.doi.org/10.2174/1566524014666141202163927 |
Print ISSN 1566-5240 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5666 |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
FoxO Transcription Factors and Regenerative Pathways in Diabetes Mellitus
Current Neurovascular Research Rheumatoid Arthritis: Cardiovascular Manifestations, Pathogenesis, and Therapy
Current Pharmaceutical Design Mediterranean Diet, Brain and Muscle: Olive Polyphenols and Resveratrol Protection in Neurodegenerative and Neuromuscular Disorders
Current Medicinal Chemistry Intracoronary Brachytherapy - Clinical State and Pathophysiological Considerations
Current Pharmaceutical Design Cardiac Morbidity in an HIV-1 Lipodystrophy Patient Cohort Expressing the TNF-α-238 G/A Single Nucleotide Gene Polymorphism
Current HIV Research Chitosan and Its Use in Design of Insulin Delivery System
Recent Patents on Drug Delivery & Formulation Peptides, Proteins and Peptide/Protein-Polymer Conjugates as Drug Delivery System
Protein & Peptide Letters Patient Impressions of Possible Catastrophic Outcomes after Diabetic Foot Ulceration
Current Diabetes Reviews Current Status on Immunoprotection of Transplanted Islets: Focus on Islet Microencapsulation
Micro and Nanosystems The Application of NMR Spectroscopy for the Study of Heart Failure
Current Pharmaceutical Design Application of Carbon Monoxide for Transplantation
Current Pharmaceutical Biotechnology Genetic and Non-genetic Determinants of Cardiovascular Disease in South Asians
Current Diabetes Reviews The Beneficial Hemodynamic Actions of SGLT-2 Inhibitors beyond the Management of Hyperglycemia
Current Medicinal Chemistry Future Perspective of Diabetic Animal Models
Endocrine, Metabolic & Immune Disorders - Drug Targets Mean Platelet Volume: A Link Between Thrombosis and Inflammation?
Current Pharmaceutical Design The Interaction of Depression and Diabetes: A Review
Current Diabetes Reviews Meet Our Regional Editor:
Protein & Peptide Letters Common Pathological Mechanisms and Risk Factors for Alzheimer’s Disease and Type-2 Diabetes: Focus on Inflammation
Current Alzheimer Research Clinical Utility of Acarbose, an α-Glucosidase Inhibitor in Cardiometabolic Disorders
Current Drug Metabolism A Review of Obesity and Body Fat Distribution and Its Relationship to Cardio-Metabolic Risk in Men and Women of Chinese Origin
Cardiovascular & Hematological Disorders-Drug Targets