Affinity-Based Methods in Drug-Target Discovery

Author(s): Gabriela Rylova, Tomas Ozdian, Lakshman Varanasi, Miroslav Soural, Jan Hlavac, Dusan Holub, Petr Dzubak, Marian Hajduch.

Journal Name: Current Drug Targets

Volume 16 , Issue 1 , 2015

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Abstract:

Target discovery using the molecular approach, as opposed to the more traditional systems approach requires the study of the cellular or biological process underlying a condition or disease. The approaches that are employed by the “bench” scientist may be genetic, genomic or proteomic and each has its rightful place in the drug-target discovery process. Affinity-based proteomic techniques currently used in drug-discovery draw upon several disciplines, synthetic chemistry, cell-biology, biochemistry and mass spectrometry. An important component of such techniques is the probe that is specifically designed to pick out a protein or set of proteins from amongst the varied thousands in a cell lysate. A second component, that is just as important, is liquid-chromatography tandem massspectrometry (LC-MS/MS). LC-MS/MS and the supporting theoretical framework has come of age and is the tool of choice for protein identification and quantification. These proteomic tools are critical to maintaining the drug-candidate supply, in the larger context of drug discovery.

Keywords: Affinity purification, drug-target discovery, immobilization, mass-spectrometry, probe, quantification.

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Article Details

VOLUME: 16
ISSUE: 1
Year: 2015
Page: [60 - 76]
Pages: 17
DOI: 10.2174/1389450115666141120110323

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