Expression, Purification and Characterization of Recombinant Toxins Consisting of Truncated Gastrin 17 and Pseudomonas Exotoxin

Author(s): Xiao-Li Feng, Xi-Lin Liu, Shi-Ying Lu, Hong-Lin Ren, Yan-Song Li, Pan Hu, Quan Wang, Weihua Tong, Dong-Ming Yan, Yu Zhou, Song Zhang, Wen Jin, Zeng-Shan Liu.

Journal Name: Protein & Peptide Letters

Volume 22 , Issue 2 , 2015

Become EABM
Become Reviewer

Graphical Abstract:


Abstract:

Gastric cancer is a major cause of mortality and morbidity around world. However the effectiveness of the current approaches to the diagnosis and treatment of gastric cancer is limited. Recombinant targeted toxins may represent a novel direction of cancer therapy. In this study, we aimed to explore whether recombinant toxins fused with the truncated forms of G17 could target to kill cancer cells by recognizing CCK2R. Four recombinant Pseudomonas toxins PE38 fused with the forward or reverse truncated forms of G17 (G14 and G13) were successfully constructed, expressed, and purified. Their characteristics were further analyzed by SDS-PAGE, western blot and indirect immunofluorescence assay. The cytotoxicity assay demonstrated that only reversely fused recombinant toxins rG14PE38 and rG13PE38 exhibited certain toxicity on several cancer cell lines, and a competition assay indicated that the binding of the reverse gastrin-endotoxin to CCK2R (+) cells may be mediated by interaction between gastrin/gastrin-like and CCK2R.

Keywords: Cytotoxicity, expression, optimization, purification, truncated gastrin 17.

Rights & PermissionsPrintExport Cite as

Article Details

VOLUME: 22
ISSUE: 2
Year: 2015
Page: [193 - 201]
Pages: 9
DOI: 10.2174/0929866521666141028214723
Price: $58

Article Metrics

PDF: 18
HTML: 1