Current Biotechnology

Pabulo Henrique Rampelotto  
Center of Biotechnology and PPGBCM
Federal University of Rio Grande do Sul
Porto Alegre, RS
Brazil

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A Thermostable Cyclodextrin Glycosyltransferase from Thermoanaerobacter sp. 5K

Author(s): Ayse Avci, Nancy N. Nichols, Badal C. Saha, Sarah E. Frazer, Michael A. Cotta, Sedat Donmez.

Graphical Abstract:


Abstract:

Cyclodextrin glycosyltransferase (CGTase) from the thermophilic anaerobe Thermoanaerobacter sp. 5K was purified and characterized. The enzyme was purified with ammonium sulfate precipitation followed by α-CD-bound, epoxy-activated Sepharose 6B affinity chromatography. Molecular weight of the purified enzyme was 70.6 kDa. The enzyme had optimal activity at 80-90°C and retained greater than 90% activity between 75°C and 95°C. Optimal pH activity was observed at 7.0, with at least 50% activity between pH 4.0 and 9.0. It was highly stable at elevated temperature, with no loss of activity after incubation at 80°C for 4 hours or at 90°C for 30 min. Km and Vmax values were 0.222 mg/mL and 0.206 mg β-CD/mL/min, respectively, with soluble starch. Amino acid composition of the enzyme was deduced from the sequence of the cloned CGTase gene. The mature enzyme has a deduced molecular weight of 75.63 kDa and contains residues conserved in the CGTase class of amylase enzymes.

Keywords: Anaerobe; cyclodextrin glycosyltransferase, cyclodextrin, Thermoanaerobacter, thermophilic extremozyme.

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Article Details

VOLUME: 3
ISSUE: 4
Year: 2014
Page: [305 - 312]
Pages: 8
DOI: 10.2174/2211550103666141027220452
Price: $58