Biochemical Characterization of a Malonyl-Specific Acyltransferase Domain of FK506 Biosynthetic Polyketide Synthase

Author(s): Yue-Yue Wang, Long-Fei Bai, Xin-Xin Ran, Xin-Hang Jiang, Hui Wu, Wei Zhang, Mei-Ying Jin, Yong-Quan Li, Hui Jiang.

Journal Name: Protein & Peptide Letters

Volume 22 , Issue 1 , 2015

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Abstract:

Acyltransferases (ATs) play an essential role in the polyketide biosynthesis through transferring acyl units into acyl carrier proteins (ACPs) via a self-acylation reaction and a transacylation reaction. Here we used AT10FkbA of FK506 biosynthetic polyketide synthase (PKS) from Streptomyces tsukubaensis YN06 as a model to study the specificity of ATs for acyl units. Our results show that AT10FkbA can form both malonyl-O-AT10FkbA and methylmalonyl-O-AT10FkbA in the self-acylation reaction, however, only malonyl-O-AT10FkbA but not methylmalonyl-O-AT10FkbA can transfer the acyl unit into ACPs in the transacylation reaction. Unlike some ATs that are known to control the acyl specificity in self-acylation reactions, AT10FkbA controls the acyl specificity in transacylation reactions.

Keywords: Acyltransferase, FK506, malonyl-CoA, methylmalonyl-CoA, polyketide synthase.

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Article Details

VOLUME: 22
ISSUE: 1
Year: 2015
Page: [2 - 7]
Pages: 6
DOI: 10.2174/0929866521666140926113322

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