Current Stem Cell Research & Therapy

Anthony Atala  
Wake Forest University School of Medicine,
Medical Center Boulevard
Winston Salem, NC 27157
USA

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Non-Viral Methods For Generating Integration-Free, Induced Pluripotent Stem Cells

Author(s): Xiao-Yue Deng, Hu Wang, Tao Wang, Xian-Tao Fang, Li-Li Zou, Zhi-Ying Li, Chang-Bai Liu.

Abstract:

Induced pluripotent stem (iPS) cells were created from mouse fibroblasts by induced expression of Yamanaka factors, Oct3/4, Sox2, Klf4, and c-Myc. This technique has quickly resulted in an exponential increase in the amount of pluripotency studies, and has provided a valuable tool in regenerative medicine. At the same time, many methodologies to generate iPS cells have been reported, and are comprised mainly of viral methods and non-viral methods. Although viral methods may not be applicable for clinical applications, various nonviral methods have been reported in recent years, including DNA vector-based approaches, transfection of mRNA, transduction of reprogramming proteins, and use of small molecule compounds. This review summarizes and evaluates these non-viral methods.

Keywords: DNA integration-free, induced pluripotent stem cells, non-viral methods.

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Article Details

VOLUME: 10
ISSUE: 2
Year: 2015
Page: [153 - 158]
Pages: 6
DOI: 10.2174/1574888X09666140923101914