Growth Inhibition of Various Human Cancer Cell Lines by Imperatorin and Limonin from Poncirus Trifoliata Rafin. Seeds
Shah Alam Siddiqui,
Sun Chul Kang.
We examined the anticancer effects of limonin and imperatorin on various human cancer cells by MTT assay, and
the results showed that imperatorin inhibited the cell growth of SNU 449 (liver cancer) and HCT-15 (colon cancer) cells in a
dose-dependent manner, while limonin had less effect. Exposure of different concentrations of limonin and imperatorin
caused morphological changes in cancer cells, but not in normal dermal fibroblast cells. Limonin and imperatorin induced
apoptotic cell death concurrent to cell cycle arrest in SNU 449 and HCT-15 cells. Limonin and imperatorin up regulated proapoptotic
protein Bax expression and down regulated anti-apoptotic protein Bcl-2 expression in a dose-dependent manner in HCT-15 and
SNU 449 cells. In conclusion, our data demonstrate that limonin and imperatorin have anticancer potential which is associated with
promoting cell apoptosis through expression of apoptosis-related proteins.
Keywords: Anticancer activity, imperatorin, limonin, Poncirus trifoliata rafin, colon cancer, liver cancer.
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