Expression and Secretion of Wild Type and Mutant GNE Proteins in Dictyostelium discoideum

Author(s): Sonam Grover, Saima Aslam, Vijeta Sharma, Ranjana Arya.

Journal Name: CNS & Neurological Disorders - Drug Targets
(Formerly Current Drug Targets - CNS & Neurological Disorders)

Volume 13 , Issue 7 , 2014

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Abstract:

GNE (UDP-N-acetylglucosamine 2-epimerase/ N-acetylmannosamine kinase) is a bifunctional enzyme which catalyzes the conversion of UDP-GlcNAc to ManNAc and ManNAc to ManNAc 6-phosphate, key steps in the sialic acid biosynthesis. Mutations in GNE lead to a neuromuscular disorder, Hereditary Inclusion Body Myopathy (HIBM). A major limitation in understanding the function of GNE is lack of recombinant full length GNE (rGNE) protein for detailed biophysical and structural characterization. In the present study, we have used Dictyostelium discoideum (Dd) as an alternate host for successful expression and secretion of functionally active form of GNE and its mutant proteins. We have generated Dd-AX3 stable cell lines harboring wtGNE or its mutants with Dd specific secretory signal sequence, PsA (prespore antigen). Upon starvation, rGNE was secreted in the medium from secretory vesicles. The rGNE was functionally active with epimerase activity (54±5.2 mU/mg) and kinase activity (66.45±3.48 mU/mg), while both epimerase and kinase activities of mutant GNE were drastically reduced. These activities were found to be statistically significant at p value < 0.05. Our study clearly demonstrates that Dd can be used as an expression host for the production of recombinant and functionally active form of GNE and its mutant proteins that can be used for biophysical characterization and structural determination of GNE to understand the pathomechanism of HIBM.

Keywords: Dictyostelium discoideum, sialic acid, muscle myopathy, secretion, UDP-N-acetylglucosamine 2-epimerase/Nacetylmannosamine kinase, hereditary inclusion body myopathy.

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Article Details

VOLUME: 13
ISSUE: 7
Year: 2014
Page: [1263 - 1272]
Pages: 10
DOI: 10.2174/1871527313666140917094905
Price: $58

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