Antimicrobial Activity of Engineered Shrimp Ovarian Peritrophin Fragments from Fenneropenaeus merguiensis

Author(s): Sujunya Anuchan, Panchalika Deachamag, Nareerat Siammai, Souwalak Phongpaichit, Wilaiwan Chotigeat.

Journal Name: Protein & Peptide Letters

Volume 22 , Issue 1 , 2015

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Graphical Abstract:


Shrimp ovarian peritrophin (SOP), a major protein in jelly layer and cortical rods, plays a role in egg protection after spawning. Previous study, sequence of SOP gene from Fenneropenaeus merguiensis (Fm-SOP) was composed of domain A and domain B. The SOP domain A contains amino acid sequences between 1-80 of Fm-SOP. The domain A had six conserved cysteines which have been found in many antimicrobial peptides. The molecular weight of purified rSOP-A protein was about 9 kDa. The SOP domain B contains amino acid sequences 81-329 of Fm-SOP while SOP-B1 was amino acid sequence 182-275 of Fm-SOP. The molecular weight of purified rHis-SOP-B and rHis-SOP-B1 protein were about 38.5 and 18.0 kDa, respectively. Antimicrobial activities of rSOP-A, rHis-SOP-B and rHis-SOP-B1 protein were investigated by liquid growth inhibition assay. Minimal Inhibition Concentration (MIC) of rSOP-A against Staphylococcus aureus, Escherichia coli, Vibrio harveyi, Candida albicans and Fusarium oxysporum were 35, 280, 280, 570 and 15 µg/mL, respectively. The MIC of rHis-SOP-B against S. aureus, V. harveyi and F. oxysporum were 30, 270 and 500 µg/mL, respectively. And the MIC of rHis-SOP-B1 against S. aureus, V. harveyi and F. oxysporum were 20, 470 and 250 µg/mL, respectively. The rHis-SOP-B and rHis-SOP-B1 (1000 µg/mL) did not show antimicrobial activity against E. coli and C. albicans. Three purified proteins were able to agglutinate V. harveyi in vitro, displayed a chitinase activity and proteinase inhibition. In addition the stability of the proteins was tested and found decrease antimicrobial activity after incubation at 50 °C for 5 h.

Keywords: Antimicrobial, protein, SOP.

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Article Details

Year: 2015
Page: [73 - 80]
Pages: 8
DOI: 10.2174/0929866521666140827114613

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