Integrase-Deficient Lentivirus: Opportunities and Challenges for Human Gene Therapy
Lentiviruses are powerful tools for gene delivery and have been widely used for the dissection of gene functions
in both replicating and quiescent cells. Recently, lentiviruses have also been used for delivering target sequences in
gene therapy. Although the lentiviral system provides sustained exogenous gene expression, serious concerns have been
raised due to its unfavorable insertion-mediated mutagenesis effect, thereby resulting in the silencing or activation of
some unexpected genes. Thus, an array of modifications of the original vectors may reduce risks. Here, we briefly review
the structure of the integrase protein, which is an essential protein for viral insertion and integration; the mechanisms of
integrase-mediated integration; and the effects of the modifications of integrase. Moreover, we discuss the advantages resulting
from integrase modifications and their future applications. Taken together, the generation of integrase-deficient
lentivirus (IDLV) not only provides us with an opportunity to reduce the risk of virus-mediated insertions, which would
improve the safety of gene therapy, but also favors gene correction and vaccine development.
Keywords: Gene delivery, gene therapy, genome manipulation, integrase-deficient lentivirus, non-integrating lentiviral vectors.
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