Abstract
Macromolecular drugs (e.g., proteins and nucleic acids) are highly environmentally liable and unstable, and their administration is strictly limited to injection. Moreover, a vast majority of macromolecules are cell membrane- impermeable, and it is a critical issue to enhance the cellular uptake efficiency for improving the treatment outcomes. Cell-penetrating peptide (CPP)-assisted strategy is promising for effective macromolecular delivery. As a case in point, CPP-mediated protein delivery has been considered as a revolutionary breakthrough. With aid of CPP, virtually all pro- teins can become cell-permeable. Generally, CPP-protein delivery works in a covalent delivery pattern, by which CPP and its cargo are linked via covalent bond. Recently, noncovalent delivery has also attracted attention for its potential application for protein delivery. In the presented work, the noncovalent pattern was demonstrated for its feasibi lity in percutaneous and nose-to-brain delivery with TAT/GFP as model drug, in comparison with the covalent method. Noncovalent CPP/protein delivery and its noninvasive application may provide a facile method for protein therapy.
Keywords: Cell-penetrating peptide, noncovalent, nose-to-brain delivery, percutaneous delivery, protein delivery, TAT.
Protein & Peptide Letters
Title:CPP-mediated Protein Delivery in a Noncovalent Form: Proof-of-Concept for Percutaneous and Intranasal Delivery
Volume: 21 Issue: 11
Author(s): Zhao Wang, Yingzhi Chen, Ergang Liu, Junbo Gong, Meong Cheol Shin and Yongzhuo Huang
Affiliation:
Keywords: Cell-penetrating peptide, noncovalent, nose-to-brain delivery, percutaneous delivery, protein delivery, TAT.
Abstract: Macromolecular drugs (e.g., proteins and nucleic acids) are highly environmentally liable and unstable, and their administration is strictly limited to injection. Moreover, a vast majority of macromolecules are cell membrane- impermeable, and it is a critical issue to enhance the cellular uptake efficiency for improving the treatment outcomes. Cell-penetrating peptide (CPP)-assisted strategy is promising for effective macromolecular delivery. As a case in point, CPP-mediated protein delivery has been considered as a revolutionary breakthrough. With aid of CPP, virtually all pro- teins can become cell-permeable. Generally, CPP-protein delivery works in a covalent delivery pattern, by which CPP and its cargo are linked via covalent bond. Recently, noncovalent delivery has also attracted attention for its potential application for protein delivery. In the presented work, the noncovalent pattern was demonstrated for its feasibi lity in percutaneous and nose-to-brain delivery with TAT/GFP as model drug, in comparison with the covalent method. Noncovalent CPP/protein delivery and its noninvasive application may provide a facile method for protein therapy.
Export Options
About this article
Cite this article as:
Wang Zhao, Chen Yingzhi, Liu Ergang, Gong Junbo, Shin Cheol Meong and Huang Yongzhuo, CPP-mediated Protein Delivery in a Noncovalent Form: Proof-of-Concept for Percutaneous and Intranasal Delivery , Protein & Peptide Letters 2014; 21 (11) . https://dx.doi.org/10.2174/0929866521666140807121903
DOI https://dx.doi.org/10.2174/0929866521666140807121903 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Chitosan Nano-encapsulation Enhances Gedunin Cytotoxicity A gainst Human Non-small-cell Lung Cancer (NCI-H292) Cell Line
Drug Delivery Letters Role of C1QBP/p32 and its Therapeutic Potential in Breast Carcinoma and other Cancers
Current Medicinal Chemistry Vasoactive Intestinal Peptide Receptors: A Molecular Target in Breast and Lung Cancer
Current Pharmaceutical Design Spectrum of Radiopharmaceuticals in Nuclear Oncology
Current Cancer Drug Targets Recent Advances in the Diagnosis and Therapy of Primary Adrenal Insufficiency
Current Medicinal Chemistry - Immunology, Endocrine & Metabolic Agents Illuminating microRNA Transcription from the Epigenome
Current Genomics Predicting Toxicity: Biomarkers and the Value of the Patient's Opinion
Current Pharmaceutical Design Smart Electrospun Nanofibers for Controlled Drug Release: Recent Advances and New Perspectives
Current Pharmaceutical Design Some Wild-Growing Plant Species from Serbia and Montenegro as the Potential Sources of Drugs
Current Pharmaceutical Design Intracellular Proton Pumps as Targets in Chemotherapy: V-ATPases and Cancer
Current Pharmaceutical Design Animal Modeling of Cancer Pathology and Studying Tumor Response to Therapy
Current Drug Targets The Role of Transmembrane Segment II in 7TM Receptor Activation
Current Molecular Pharmacology Biosafety Challenges for Use of Lentiviral Vectors in Gene Therapy
Current Gene Therapy The Molecular Targets of Antitumor 2-deoxycytidine Analogues
Current Drug Targets Human Endometrial and Ovarian Cancer Cells: Histone Deacetylase Inhibitors Exhibit Antiproliferative Activity, Potently Induce Cell Cycle Arrest, and Stimulate Apoptosis
Current Medicinal Chemistry The Metabolism of Anticancer Drugs by the Liver: Current Approaches to the Drug Development Process
Current Drug Metabolism Biomedical Applications of Protein Microarrays
Current Medicinal Chemistry The Different Roles of The Channel-Kinases TRPM6 and TRPM7
Current Medicinal Chemistry Solvent-Free and Self-Catalyzed Three-Component Synthesis of Diversely Substituted Pyrazolo[1,4]thiazepinones of Potential Antitumor Activity
Current Organic Synthesis Melanoma Differentiation Associated Gene-7 (mda-7)/ Interleukin-24 (IL-24), mda-7/IL-24: Current Perspectives on a Unique Member of the IL-10 Family of Cytokines
Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry