Solid Lipid Nanoparticles Loaded with Fluorescent-labelled Cyclosporine A: Anti-Inflammatory Activity In Vitro

Author(s): Marina Gallarate, Loredana Serpe, Federica Foglietta, Gian Paolo Zara, Susanna Giordano, Elena Peira, Daniela Chirio, Luigi Battaglia.

Journal Name: Protein & Peptide Letters

Volume 21 , Issue 11 , 2014

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Abstract:

FMOC-isocyclosporine A, a fluorescent labeled cyclosporine A, was encapsulated in solid lipid nanoparticles (SLN) prepared by the coacervation technique, and its anti-inflammatory activity was evaluated. The anti-inflammatory activity of the fluorescent labelled molecule, measured as inhibition of TNF-α secretion, is similar to the native one. SLN were compared to commercial formulations, through measurement of cytokine release and drug uptake in rat peripheral blood mononuclear cells. Drug-loaded SLN inhibit TNF-α secretion in a lower extent than commercial formulations, probably due to a lower uptake by the cells, but the increase of IL-10 secretion caused by the lipid matrix itself makes this formulation interesting for its anti-inflammatory activity.

Keywords: Coacervation, cyclosporine A, cytokines, FMOC, inflammation, solid lipid nanoparticles.

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Article Details

VOLUME: 21
ISSUE: 11
Year: 2014
Page: [1157 - 1162]
Pages: 6
DOI: 10.2174/0929866521666140806164410
Price: $58

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