Setting a Colorimetric Assay with MTT for Assessment of Trichomonicidal Activity
Idalia Sariego, Lianet Monzote and Lazara Rojas
Affiliation: Departamento de Parasitologia, Instituto de Medicina Tropical “Pedro Kouri”, Autopista Novia del Mediodia, Km 6½, La Habana, Cuba.
Keywords: Colorimetric method, drug susceptibility assay, MTT assay, Trichomonas vaginalis.
Trichomonas vaginalis infection is associated with important problems of public health, including the
spreading of other sexual transmitted infections. The existence of clinical resistant isolates metronidazole and tinidazole,
the drugs approved for the treatment of trichomoniasis, points to the necessity of continue searching for trichomonicidal
substances. Here we optimize a colorimetric assay with MTT to assess trichomonas viability. The absence of ascorbic acid
and cysteine in the culture medium was indispensable to perform the assay, as these compounds spontaneously reduce the
MTT. Linearity of absorbance was verified versus trichomonas counts. Medium inhibitory concentration of metronidazole
was determined using the sigmoidal Emax model, by comparing the absorbance of test cultures with controls. The
obtained value was in the range of published data. The test would be used for the evaluation of trichomonicidal activity of
chemical compounds and natural products.
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