A Screening Method for Potential Anti-pancreatic Cancer Candidate Compounds Based on Hsp90-Cdc37 Interaction Model in vitro

Author(s): Hai Zhang, Sen Sun, Qingshan Chen, Guoqing Zhang, Zhenqing Zhang, Duxin Sun, Wenpeng Zhang.

Journal Name:Letters in Drug Design & Discovery

Volume 12 , Issue 1 , 2015

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Abstract:

The Hsp90/Cdc37 (heat shock protein 90; cell division cycle 37) interaction is a promising target for anticancer candidate drug development. The purpose of this research is to establish a screening method for anti-cancer candidate compounds based on the Hsp90/Cdc37 interaction model in vitro by Split Renilla luciferase protein fragment-assisted complementation bioluminescence technology. The parameters influencing the protein binding in this model were optimized, including protein concentration, reaction time, and substrate concentration. The optimal condition was as follows: protein concentration 2µM, the ratio of Hsp90 and Cdc37 1:1, reaction time 10 min, and the GLO® substrate (Promega, Madison, WI, USA) dilution 1:100. The screening model was further validated by some known compounds blocking the Hsp90/Cdc37 interactions. It was proved that this method is rapid and sensitive and could be used for screening of antipancreatic cancer candidate compounds.

Keywords: Anti-cancer, Cdc37, a screening method, Hsp90, Hsp90/Cdc37 interaction, protein interaction model in vitro, SRLPFAC.

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Article Details

VOLUME: 12
ISSUE: 1
Year: 2015
Page: [66 - 71]
Pages: 6
DOI: 10.2174/1570180811666140725185235

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