Current Protein & Peptide Science

Ben M. Dunn  
Department of Biochemistry and Molecular Biology University of Florida
College of Medicine, P.O. Box 100245, Gainesville
Florida, FL 32610-0245


The Organelle-focused Proteomes and Interactomes in Rice

Author(s): Lili Liu, Li Jiang, Ming Chen.


Protein subcellular localization has been a long-standing key problem in investigating proteins’ function, which provides important clues for revealing their functions and aids in understanding their interactions with other biomolecules at the cellular level. Here, we systematically defined the organelle-focused proteomes and interactomes in Oryza sativa. A total of 83.42% of the whole rice proteome obtained their subcellular localizations based on manual annotation, manual adjustment and predictors’ cross validation. The final organelle-focused interactomes were located in nine organelles. Furthermore, we discussed the cross talk bias between different organelles and the function organization accounting for nine organelles. Motif analysis illustrated the protein interaction bias in different organelles to implement certain biology functions. We exemplified the connection between functions and the overrepresented motifs in the organelle-focused interactomes and exemplified how to infer the functions of unknown proteins via expanding the overrepresented motifs.

Keywords: Function enrichment analysis, motif analysis, organelle-focused interactome, organelle-focused proteome, rice.

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Article Details

Year: 2014
Page: [583 - 590]
Pages: 8
DOI: 10.2174/1389203715666140724084331
Price: $58