Structural Characterization, Homology Modeling and Docking Studies of ARG674 Mutation in MyH8 Gene Associated with Trismus-Pseudocamptodactyly Syndrome
Munazzah Tasleem, Romana Ishrat, Asimul Islam, Faizan Ahmad and Md. Imtaiyaz Hassan
Affiliation: Centre for Interdisciplinary Research In Basic Sciences, Jamia Millia Islamia, Jamia Nagar, New Delhi 10025, India.
Keywords: Docking, homology modeling, MyH8 gene, myosin, protein-protein interaction and trismus-pseudocamptodactyly
Trismus-pseudocamptodactyly (TPS) syndrome is a musculoskeletal disorder, caused by mutation in the perinatal
MyH8 gene, leading to the incomplete opening of mouth and camptodactyly of fingers upon dorsiflexion of the
wrist. MyH8 gene is relevant for muscle regulation, and it plays a significant role in muscle motor function, the hydrolysis
of ATP, that is essential for the production of force for muscle movement. To understand the structural basis of TPS, we
utilize a large number of software packages to estimate how the Arg674Gln mutation would affect the structure and stability
of MyH8 gene product. The motor domain of MYH8 was modeled in order to know what are the interactions altered in
the mutant protein. Further, we docked the ATP in the nucleotide binding region of both wild type and Arg674Gln mutant
to understand the mechanism of action. Our strategy may be helpful for understanding the mechanism of regulation of
muscle movements and the role of Arg674Gln mutation in TPS.
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