Letters in Drug Design & Discovery

G. Perry
University of Texas
San Antonio, TX
USA
Email: lddd@benthamscience.org

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Structural Characterization, Homology Modeling and Docking Studies of ARG674 Mutation in MyH8 Gene Associated with Trismus-Pseudocamptodactyly Syndrome

Author(s): Munazzah Tasleem, Romana Ishrat, Asimul Islam, Faizan Ahmad, Md. Imtaiyaz Hassan.

Graphical Abstract:


Abstract:

Trismus-pseudocamptodactyly (TPS) syndrome is a musculoskeletal disorder, caused by mutation in the perinatal MyH8 gene, leading to the incomplete opening of mouth and camptodactyly of fingers upon dorsiflexion of the wrist. MyH8 gene is relevant for muscle regulation, and it plays a significant role in muscle motor function, the hydrolysis of ATP, that is essential for the production of force for muscle movement. To understand the structural basis of TPS, we utilize a large number of software packages to estimate how the Arg674Gln mutation would affect the structure and stability of MyH8 gene product. The motor domain of MYH8 was modeled in order to know what are the interactions altered in the mutant protein. Further, we docked the ATP in the nucleotide binding region of both wild type and Arg674Gln mutant to understand the mechanism of action. Our strategy may be helpful for understanding the mechanism of regulation of muscle movements and the role of Arg674Gln mutation in TPS.

Keywords: Docking, homology modeling, MyH8 gene, myosin, protein-protein interaction and trismus-pseudocamptodactyly syndrome.

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Article Details

VOLUME: 11
ISSUE: 10
Year: 2014
Page: [1177 - 1187]
Pages: 11
DOI: 10.2174/1570180811666140717190217
Price: $58