Effect of Nutrient Medium, Phytohormones and Elicitation Treatment on in-vitro Callus Culture of Bacopa monniera and Expression of Secondary Metabolites
Aziz Ahmed, Mahfoozur Rahman, Tamboli E. Tajuddin, Tanwir Athar, Mhaveer Singh, Madhukar Garg and Sayeed Ahmad
Affiliation: Abhilashi College of Pharmacy, Himachal Pradesh, India.
Keywords: Bacopa monniera, callus culture, elicitation, bacosides.
Bacopa monniera (L.) Wettst. (Scrophulariaceae) is a small creeping herb with numerous branches, small
oblong leaves, and light purple flowers. The entire plant is medicinally important as it contains triterpene saponins.
Important saponins include Bacosides A1, bacosides A3, bacosaponins A, B, C, D, E & F. The constituents responsible for
Bacopa’s cognitive effects are bacosides A and B. In the present investigation, attempt has been made for the development
of the novel in vitro methods of propagation. Explants were aseptically cultured on Murashige and Skoog (MS) medium,
and plant medium supplemented with different concentrations of auxins and cytokinins like 2, 4-Dichlorophenoxyacetic
acid (2,4-D), Indole Acetic Acid (IAA), Naphthalene Acetic Acid (NAA), 6-Benzyl Adenine (6-BA) and Kinetin,
individually as well as in combinations. Calli were also subjected to elicitation treatment. After 6th passage, calli were
subjected to extraction and phytochemical screening. Out of various hormonal combinations, the MS medium supplemented
with 6 BA (2ppm) + IAA (1ppm) was found to be the most effective for induction of Yellowish green callus, within 15
days. The preliminary phytochemical analysis of callus showed the presence of Alkaloids, Carbohydrates, Phenolic
compounds and Tannins, Flavonoids, Proteins and free amino acids, Saponins, Steroids and Terpenoids.
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