Phenolic Compounds and Metals in Tommy Atkins Mango (Mangifera indica L) Peels
Cristiane R. de Araujo, Priscilla de S. Botelho, Tania M.S. da Silva, Maria Ines S. Maciel, Enayde de A. Melo, Sean Ryan and Robert E. Smith
Affiliation: US FDA, 11510 W 80th St., Lenexa, KS 66214, USA.
Polyphenolic compounds and metals in lyophilized mango peels were identified and antioxidant capacities of
these extracts were evaluated using in vitro assays. The concentrations of calcium, potassium, sodium, phosphorus,
copper, iron, magnesium, manganese, zinc, cadmium and nickel were 2177, 7149, 7.416, 542.6, 3.227, 12.78, 1270, 23.47,
3.833, 0.040 and 1.125, respectively. To obtain the phenolics, two extracts were obtained (Ext-5 and Ext-7) using 80%
methanol and 80% acetone, at 25 and 37.5 °C with stirring for 30 and 60 min, respectively. The extracts were applied to
solid-phase extraction (SPE) cartridges and analyzed by liquid chromatography (HPLC-diode array detector) to identify
and quantify polyphenols. The DPPH and ABTS radicals scavenging capacity was evaluated in these extracts. Phenolic
acids, flavonoids, and one xanthone were identified in both extracts, independently of the conditions used in the extraction
process. Compounds identified include syringic and sinapic acids, as well as taxofolin, isoquercetin, and mangiferin. The
results suggest that the extracts’ capacity to sequester DPPH (1,1-diphenyl-2-picrylhydrazyl) and ABTS (2,2’-azino-bis(3-
ethylbenzothiazoline-6-sulphonic acid)radicals was significant and may result from either the high concentrations of some
of the identified polyphenols or synergy with polyphenols at lower concentrations.
Keywords: ABTS, Antioxidant capacity, antioxidants, DPPH, flavonoids, free radicals scavenging capacity, mango, metals,
mango peel, phenolic acids, phenols.
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