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Current Nanoscience
ISSN (Print): 1573-4137
ISSN (Online): 1875-6786
Epub Full Text Article
DOI: 10.2174/1573413710666140714171448      Price:  $95









Study of the Interaction between Graphene Oxide and Surface-confined Biomolecules to Develop New Kind of Biosensors

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Author(s): Xiaoxi Li, Peng Miao, Limin Ning, Tao Gao, Zonghuang Ye and Genxi Li
Abstract:
In this work, we have studied the interaction between graphene oxide (GO) and the biomolecules immobilized on an electrode surface, which has been further employed to develop new kind of biosensors by fabricating gamma-glutamyltransferase (γ-GGT) sensor as an example. Specifically, glutathione (GSH) is firstly immobilized on the surface of a gold electrode, and GO nanosheet is introduced to recognize the charge change of GSH caused by the catalysis of γ-GGT. Then, the introduction of reductant and Ag+ induces silver deposition and partial reduction of GO, forming GO-AgNPs complex for electrochemical readout. Since this complex may have excellent electric conductivity and the solid-state voltammetry of Ag/AgCl can provide a well-defined symmetrically sharp silver stripping peak, a sensitive electrochemical biosensor for the detection of γ-GGT is thus developed. Moreover, the experimental results obtained in this work indicate that GO itself can serve as a general recognition element for biosensor fabrication and GO-AgNPs can be a promising material in a general sensing platform, so such kind of more biosensors can be developed in the future.
Keywords:
Electrochemical assay, gamma-glutamyltransferase, graphene oxide, in situ reduction, silver nanoparticles, solidstate voltammetry
Affiliation:
Department of Biochemistry and State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, P R China.