Interactive Effect of Agitation Speed and Aeration Rate on Heat Stable β-Carotene Production from Mucor Azygosporus using Deprotenized Waste whey Filtrate in Stirred Tank Reactor
Wamik Azmi, Meenu Thakur, Azhar Javed and Neetika Thakur
Interactive effects of aeration rate and agitation speed on β-carotene production by Mucor azygosporus using waste whey filtrate in a laboratory fermenter were studied. The maximum β-carotene concentration (1045 µg/g dcw) was obtained at low agitation speed (150 rpm) and moderate aeration rate (1.0 vvm) after 72 h of incubation with a biomass of 7.9 g/L. The agitation and aeration provided better homogeneity in the culture medium with greater availability of nutrients and oxygen leading to a 2.8-fold increase in β-carotene production from that in the shake flask.
β-carotene, M. azygosporus, fermentation, whey filtrate, stirred tank reactor
Department of Biotechnology Himachal Pradesh University, Summer Hill, Shimla - 171005, India.