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Current Stem Cell Research & Therapy
ISSN (Print): 1574-888X
ISSN (Online): 2212-3946
Epub Full Text Article
DOI: 10.2174/1574888X09666140710115356      Price:  $95

VEGF, BMP-7, MatrigelTM, Hyaluronic Acid, in Vitro Cultured Chondrocytes and Trephination for Healing of the Avascular Portion of the Meniscus. An Experimental Study in Sheep

Author(s): Francisco Forriol, Umile Giuseppe Longo, Julio Duart, Purification Ripalda, Javier Vaquero, Mattia Loppini, Giovanni Romeo, Stefano Campi, Wasim Khan, Andrea Onetti Muda and Vincenzo Denaro
Objective: The aim of this study was to evaluate the effects of VEGF, BMP-7, MatrigelTM, hyaluronic acid, in vitro cultured chondrocytes and trephination in an animal model to improve healing of avascular meniscal tears. Methods: A longitudinal tear was produced in the avascular portion of the meniscus of 24 sheeps. Each tear was treated with trephination technique and suture. The animals were divided into 6 groups to receive a different treatment: control (I); VEGF, BMP-7, MatrigelTM, hyaluronic acid, in vitro cultured chondrocytes. Meniscal samples were collected after 8 weeks, and the samples were analyzed by histology, immunohistochemistry, and histomorphometry. Results: At the histological examination, Group IV and VI showed a partial closure of the meniscal lesion, whereas Group I, II, III, and V did not show any evidence of healing. In the group IV, the healed tissue represented the 22.95% of the lesion area. In the group VI, the healed tissue represented the 43.75% of the lesion area. Conclusions: Autologous chondrocytes and BMP-7 associated with trephination and suture techniques enhanced healing process of meniscal tears in the avascular inner third of the meniscus in ovine model
BMP-7, cell-therapy, growth factors, healing, meniscus, repair
Department of Orthopaedic and Trauma Surgery Campus Bio-Medico University Via Alvaro del Portillo, 200 00128 Trigoria, Rome, Italy