Effects of Perfluorocarbon Dodecafluoropentane (NVX-108) on Cerebral Microvasculature in the Healthy Rat

Author(s): Paula F. Moon-Massat, Rania Abutarboush, Georgina Pappas, Ashraful Haque, Chioma Aligbe, Francoise Arnaud, Charles Auker, Richard McCarron, Anke Scultetus.

Journal Name: Current Drug Discovery Technologies

Volume 11 , Issue 3 , 2014

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Abstract:

NVX-108, a dodecafluoropentane-based perfluorocarbon (PFC) emulsion, has therapeutic potential as an oxygen- carrying fluid for emergency medical treatment for traumatic brain injury (TBI) and hemorrhagic shock. Potential cerebral vasoactive properties were assessed by directly measuring pial arteriolar vessel diameters before and after a 30 minute intravenous (IV) infusion of 1.0 ml/kg (high dose [H]) or 0.25 ml/kg (low dose [L]) NVX-108 compared to 2.0 ml/kg Saline (control) in healthy anesthetized rats (N = 6/group). Results showed that post-infusion vessel diameters for small (< 50 µm) and medium (50-100 µm)-sized pial arterioles were significantly (p < 0.05) narrower after only the NVX- 108 H infusion although this vasoconstriction was not statistically significant when analyzed as a percentage change in these vessels. Pial arteriolar vessel diameters were not significantly different for mean value or percentage change after either NVX-108 L or Saline infusions. There were no significant post-infusion changes from baseline in systolic, mean or diastolic blood pressures after any of the treatments although post-infusion blood pressure was statistically higher in the NVX-108 L group compared to NVX-108 H and Saline groups. Arterial blood gases, methemoglob in and lactate were not different from baseline or among groups. No adverse events were observed at either dose of NVX-108. In conclusion, neither 0.25 nor 1.0 ml/kg NVX-108 caused vasoconstriction in cerebral pial arterioles of healthy rats nor resulted in blood pressure changes; the compound should be considered for further investigation for TBI therapy.

Keywords: Cerebral microcirculation, dodecafluoropentane, intravital microscopy, oxygen therapeutic, perfluorocarbon, perflutren, pial arteriolar vessel diameter, rat, traumatic brain injury, vasoconstriction.

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Article Details

VOLUME: 11
ISSUE: 3
Year: 2014
Page: [220 - 226]
Pages: 7
DOI: 10.2174/1570163811666140709110301
Price: $58

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