Florfenicol is a synthetic and broad-spectrum antibiotic and developed for veterinary use. In this study, onestep
membrane-based competitive colloidal gold-based immunochromatographic formats for the rapid detection of
florfenicol were developed. The quality of colloidal gold particles was monitored by UV-vis spectroscopy. For the
conjugation of colloidal gold and antibody, 24 μg/mL of antibody was confirmed to the optimal amount for stabilization
of colloidal gold. With the prepared colloidal gold immunochromatography test strip was determined the standard
florfenicol solution. The results demonstrated a detection limit of florfenicol was approximately 1.0 μg/mL. The study
showed that the strip had a high sensitivity to florfenicol and without cross-reaction for other antibiotics. The results of
80 animal samples tests confirmed that 0 sample was higher than the detection limits by liquid chromatography-mass
spectrometry (LC-MS). There was evidence to suggest that colloidal gold particles and GAB gold-labeled antibody
were synthesized successfully. The prepared colloidal gold immunochromatography strip could be used for preliminary
screening of florfenicol.
Keywords: Colloidal gold, florfenicol, immunochromatography, LC-MS.
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