Effect of Sulfonamides on the Electrophysical Properties of Bacterial Cells
The effect of sulfonamide drugs on the electrophysical properties of Escherichia coli strains B-878 and K-12
was investigated by the example of sulfacetamide sodium (sulfacyl sodium). Substantial changes in the orientational spectra
of cell suspensions incubated with various concentrations of sulfacetamide sodium occurred only at the first five frequencies
of the orienting electric field (740–2800 kHz). Sulfacetamide sodium at 0.08 µg/ml changed the magnitude of
the electro-optical signal of the suspensions. A study of the time course of sulfacetamide sodium effect on the cells
showed that the signal magnitude decreased by 57% of the no-drug control after 5 min of exposure to the drug and that on
subsequent exposure, the signal magnitude remained almost unchanged (changes no greater than 5% of the no-drug control).
The spectral changes differed significantly for the sensitive and resistant strains. The orientational spectral changes
induced by sulfonamides may be useful for testing the resistance of bacteria to this family of drugs.
Keywords: Bacterial cells, electro-optical analysis, Escherichia coli, sulfonamides, sensitivity.
Rights & PermissionsPrintExport