Studies on Growth Kinetics of Serratia marcescens VITSD2 and Optimization of Fermentation Conditions for Serratiopeptidase Production
C. Subathra Devi, Shah Alam, Suraj Kumar Nag, S. Jemimah Naine, V. Mohanasrinivasan and B. Vaishnavi
Affiliation: Industrial Biotechnology Division, School of Biosciences and Technology, VIT University, Vellore, Tamil Nadu, India.
Keywords: Anti-inflammatory agents, inflammation, optimisation, peptidases, Serratia marcescens VITSD2, serratiopeptidase.
Serratia is one of the most important groups of bacteria which produces proteolytic enzymes effectively and
known to possess anti- inflammatory properties. The main focus of the current study was to optimize the culture conditions
of Serratia marcescens VITSD2 for the mass production of serratiopeptidase. Effect of various nutritional and environmental
factors were analysed and optimized. Among the different carbon and nitrogen sources tested, mannose and
soya bean meal was found to be the best with enzyme activity of 1391 units /mL and 1800 U/mL respectively. The enzyme
showed an optimum activity of 1668 U/mL at pH-8 and 1500 U/mL at 25ºC. Maximum peptidase production during
fermentation was obtained after 24 h incubation with 1% inoculum in the medium at 25ºC and yielded 1668 U/mL. Lysine
stimulated the production of peptidase and the yield obtained was 2410U/mL. Growth curve analysis was done. Maximum
serratiopeptidase production was detected after 24 h incubation with 2155 units/mL and cell density of 2.4g/100mL.
Hence the observation of the present study clearly indicates that the yield of Serratiopeptidase was found to be maximum
by varying the cultural conditions.
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