Current Approaches to Glycoprotein Analysis

Author(s): Andrew K. Hagan, Maomao Wang, Li Liu.

Journal Name:Protein & Peptide Letters

Volume 21 , Issue 10 , 2014

Abstract:

Glycoproteins are becoming increasingly relevant in therapeutics, including tissue plasminogen activator for the treatment of myocardial infarction and strokes, erythropoietin for anemia and various monoclonal antibody–based treatments for cancer. Protein N- and O-glycosylation is perhaps the most crucial and immensely complex posttranslational modification that proteins undergo, and its characterization presents a major challenge. This review will discuss current techniques for the characterization of glycoproteins, with a focus on therapeutic glycoproteins where available. The crucial analytical techniques, such as high-performance liquid chromatography (HPLC), capillary electrophoresis (CE), and mass spectrometry (MS) will be described, alongside the necessary chemical labeling methods for sensitive detection. The well-established chemical and enzymatic methods for oligosaccharide release from proteins will be discussed, as will more modern methods based on exhaustive protein hydrolysis with non-specific proteases.

Keywords: Analytical techniques, chemical derivatization, glycosylation, HPLC, mass spectrometry, proteases, therapeutic glycoproteins.

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Article Details

VOLUME: 21
ISSUE: 10
Year: 2014
Page: [986 - 999]
Pages: 14
DOI: 10.2174/0929866521666140626095752
Price: $58

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