Current Tissue Engineering

Herman S. Cheung  
Department of Biomedical Engineering
University of Miami
Miami, FL 33101
USA

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Association of β-tubulin, F-box/Leucine-Rich Repeat Protein 14, and Type 1 Procollagen C-peptide in Bovine Periosteal Cells

Author(s): Mari Akiyama.

Abstract:

The noncollagenous proteins UACA, EXOSC9, and TMX2 were previously identified in bovine periosteal cells with a combination of mass spectrometry and immunohistochemistry. In this study, the proteins β-tubulin, F-box/leucinerich repeat protein 14 (FBXL14), and type 1 procollagen C-peptide were investigated in these cells. Peptides of β-tubulin and FBXL14 were detected with liquid chromatography tandem mass spectrometry, with protein sequence coverages of 2% and 3%, respectively. A two-dimensional protein electrophoretic map of the culture supernatant of bovine periosteal cells suggested that TMX2 was close to type 1 procollagen C-peptide in the cell aggregate. Therefore, an immunohistochemical analysis of β-tubulin, FBXL14, and type 1 procollagen C-peptide was performed. The bovine periosteal cells were positive for β-tubulin, FBXL14, and type 1 procollagen C-peptide. The noncollagenous proteins β-tubulin and FBXL14 were thus identified, like UACA, EXOSC9, and TMX2, in bovine periosteal cells. Type 1 procollagen C-peptide was only expressed in a subset of cells, whereas type 1 collagen was expressed ubiquitously. It is possible that type 1 procollagen C-peptide is associated with those noncollagenous proteins. This study demonstrates the utility of a technique combining MS and immunohistochemistry.

Keywords: β-tubulin, bovine periosteal cells, F-box/leucine-rich repeat protein 14, scaffold-free, type 1 procollagen C-peptide.

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Article Details

VOLUME: 3
ISSUE: 1
Year: 2014
Page: [2 - 6]
Pages: 5
DOI: 10.2174/2211542003666140617000141