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Current Drug Delivery
ISSN (Print): 1567-2018
ISSN (Online): 1875-5704
Epub Full Text Article
DOI: 10.2174/1567201811666140616160237      Price:  $95









Preparation and in-vitro transfection efficiency evaluation of modified cationic liposome-polyethyleneimine-plasmid nanocomplexes as a novel gene carrier

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Author(s): Asma Mahmoudi, Reza Kazemi Oskuee, Mohammad Ramezani and Bizhan Malaekeh-Nikoue
Abstract:
Liposome-linear polyethyleneimine (PEI)-DNA nanocomplexes have shown to be an effective non-viral gene delivery vector. In the present study, we tried to improve the transfection efficiency of these nanocomplexes by liposome modification. For this purpose, the lipopolymer prepared by the conjugation of hexylacrylate to the PEI. Liposomes comprising of lipopolymer and DOTAP (1.2-DiOleoyl-3-Trimethyl Ammonium-Propane) were prepared and extruded through polycarbonate filters to obtain desired size. The 2.5, 25 and 250 KDa molecular weights of linear PEI have been used in order to prepare modified liposome-PEI-DNA nanocomplexes. Three C/P ratios of each nanocomplexes were premixed. Size, zeta potential and the DNA condensation ability of these complexes were determined separately, and at the end, the transfection efficiency and cell cytotoxicity of prepared vectors were evaluated on Neuro2A cell line. Mean particle size of all these nanocomplexes was lower than 220 nm with surface charge of 17.5 to 25.9 mV. The lipopolyplexes (comprising of modified liposome:PEI:DNA), modified liposome (as lipoplex) and PEI 250KDa (as polyplex) showed the highest transfection efficacy. This activity was amplified by increasing of carrier to plasmid (C/P) ratio. In addition, the metabolic activity of prepared vectors was 80-100% of control group. In conclusion, the prepared lipopolyplexes showed high ability to enhance gene transfer
Keywords:
Liposomes, Lipopolyplexes, Non-viral vector, Polyethyleneimine, Transfection efficiency, Gene delivery
Affiliation:
School of Pharmacy, Mashhad University of Medical Sciences,Mashhad, Iran