Preparation and in-vitro Transfection Efficiency Evaluation of Modified Cationic Liposome-polyethyleneimine-plasmid Nanocomplexes as a Novel Gene Carrier
Asma Mahmoudi, Reza Kazemi Oskuee, Mohammad Ramezani and Bizhan Malaekeh-Nikoue
Affiliation: School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, P.O. Box: 91775-1365, Iran.
Keywords: Gene delivery, Liposomes, Lipopolyplexes, Non-viral vector, Polyethyleneimine, Transfection efficiency.
Liposome-linear polyethyleneimine (PEI)-DNA nanocomplexes have shown to be effective non-viral gene delivery
vectors. In the present study, we tried to improve the transfection efficiency of these nanocomplexes by liposome
modification. For this purpose, the lipopolymer was prepared by the conjugation of hexylacrylate to the PEI. Liposomes
comprising lipopolymer and DOTAP (1.2-DiOleoyl-3-Trimethyl Ammonium-Propane) were prepared and extruded
through polycarbonate filters to obtain the desired size. The 2.5, 25 and 250 KDa molecular weights of linear PEI have
been used in order to prepare modified liposome-PEI-DNA nanocomplexes. Three C/P ratios of each nancomplex were
premixed. Size, zeta potential and the DNA condensation ability of these complexes were determined separately, and in
the end, the transfection efficiency and cell cytotoxicity of prepared vectors were evaluated on Neuro2A cell line. Mean
particle size of all of these nanocomplexes was lower than 220 nm with surface charge of 17.5 to 25.9 mV. The lipopolyplexes
(comprising modified liposome:PEI:DNA), modified liposome (as lipoplex) and PEI 250KDa (as polyplex)
showed the highest transfection efficacy. This activity was amplified by increase carrier to plasmid (C/P) ratio. In addition,
the metabolic activity of prepared vectors was 80-100% for control group. In conclusion, the prepared lipopolyplexes
showed high ability to enhance gene transfer.
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