Conjugate vaccines have been the most successful way to prevent and control deadly bacterial infections; however their
production is complex and tedious. The manufacturing process of these large molecules consists of a biological production and extraction
of polysaccharide antigen and protein carrier and its chemical conjugation. Herein a simplified manufacturing process is described where
the conjugate vaccine is completely produced in bacterial cells. The polysaccharide antigen and the protein carrier are expressed recombinantly
in E. coli cells and linked covalently by an enzymatic process. This novel biological production system leads to improved products
and opens up the possibility to develop a wide variety of novel vaccines.
Keywords: Bacterial vaccines, bioconjugates, chemical conjugation, conjugate vaccines, glycosylation, production of glycoconjugates in E. coli.
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