Seven coumarin derivatives were screened for their inhibitory effect on laccase utilizing ABTS and L-DOPA as
substrates. 2-[(4-Methyl-2-oxo-2H-1-benzopyran-7-yl)oxy]acetic acid (E)-2-[(dihydroxyphenyl)methylene]hydrazides
were proven to be stronger inhibitors than corresponding thiosemicarbazides when ABTS [2,2'-azinobis(3-
ethylbenzothiazoline-6-sulfonic acid)] was used as a substrate. In kinetic experiments mixed type inhibition was determined
for 2-[(4-methyl-2-oxo-2H-1-benzopyran-7-yl)oxy]acetic acid (E)-2-[(2,5-dihydroxyphenyl)methylene]hydrazide
and (E)-2-[(3,4-dihydroxyphenyl)methylene]hydrazide. In reaction of L-DOPA (L-3,4-dihydroxyphenylalanine) oxidation
catalyzed by laccase none of the tested compounds has shown inhibitory effect.
Keywords: Coumarins, laccase activity, inhibition, ABTS, L-DOPA.
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